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	Provedor de dados Anais da ABC (AABC) (1) Genet. Mol. Biol. (1) Autor Marins,Luis F. (2) Almeida,Daniela V. (1) Alves-Costa,Fernanda A. (1) Figueiredo,Márcio A. (1) Folch,Josep M. (1) Lanes,Carlos F.C. (1) Levy,Jose A. (1) Sanchez,Armand (1) Wasko,Adriane P. (1) Mais... Palavra-chave Growth hormone (2) CDNA (1) Differential display (1) GH (1) Phylogeny (1) RACE (Rapid Amplification of cDNA Ends) (1) Transgenic (1) Zebrafish (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A comparative expression analysis of gene transcripts in brain tissue of non-transgenic and GH-transgenic zebrafish (Danio rerio) using a DDRT-PCR approach Anais da ABC (AABC) Alves-Costa,Fernanda A.; Figueiredo,Márcio A.; Lanes,Carlos F.C.; Almeida,Daniela V.; Marins,Luis F.; Wasko,Adriane P.. The presence of higher level of exogenous growth hormone (GH) in transgenic animals could lead to several physiological alterations. A GH transgenic zebrafish (Danio rerio) line was compared to nontransgenic (NT) samples of the species through a DDRT-PCR approach, with the goal of identifying candidate differentially expressed transcripts in brain tissues that could be involved in GH overexpression. Densitometric analyses of two selected amplification products, p300 and ADCY2, pointed to a significant lower gene expression in the transgenic zebrafish (104.02 ± 57.71; 224.10 ± 91.73) when compared to NT samples (249.75 ± 30.08; 342.95 ± 65.19). The present data indicate that p300 and ADCY2 are involved in a regulation system for GH when high circulating... Tipo: Info:eu-repo/semantics/article Palavras-chave: Differential display; GH; Growth hormone; Transgenic; Zebrafish. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200017 A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis Genet. Mol. Biol. Marins,Luis F.; Levy,Jose A.; Folch,Josep M.; Sanchez,Armand. The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that... Tipo: Info:eu-repo/semantics/article Palavras-chave: Growth hormone; CDNA; RACE (Rapid Amplification of cDNA Ends); Phylogeny. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300013 Registros recuperados: 2 Primeira ... 1 ... Última

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